Design superiority of palindromic DNA sites for site-specific recognition of proteins: Tests using protein stitchery
Changmoon Park, Judith L. Campbell, William A. Goddard III
1993Proc. Natl. Acad. Sci. U.S.A., 90(11), 4892-489622cited
Abstract
Using protein stitchery with appropriate attachment of cysteines linking to either C or N termini of the basic region of the v-Jun leucine zipper gene-regulatory protein, we constructed three dimers--pCC, pCN, and pNN. All three bind specifically to the appropriately rearranged DNA recognition sites for v-Jun: ATGAcgTCAT, ATGAcgATGA, and TCATcgTCAT, respectively (K_d, approximately 4 nM at 4ºC). Results of DNase I footprinting provide strong support for bent recognition helices in leucine zipper protein-DNA complexes. Comparison of the results for pCC and pNN with those for pCN shows the design superiority of palindromic sequences for protein recognition.
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Park, C., Campbell, J. L., & III, W. A. G. (1993). Design superiority of palindromic DNA sites for site-specific recognition of proteins: Tests using protein stitchery. *Proc. Natl. Acad. Sci. U.S.A.*, *90*(11), 4892-4896. https://doi.org/10.1073/pnas.90.11.4892